Home Biology Biotechnology and Its Application Previous Year Questions The colonies of recombinant bacteria appear …
Biology Biotechnology and Its Application Previous Year Questions Single Correct MCQ
Published on: August 21, 2026

The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of

A
Non-recombinant bacteria containing b-galactosidase.
B
Insertional inactivation of a-galactosidase in non-recombinant bacteria.
C
Insertional inactivation of a-galactosidase in recombinant bacteria.
D
Inactivation of glycosidase enzyme in recombinant bacteria.

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Text Solution

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The correct answer is:
C

In recombinant bacteria, the inserted DNA disrupts the lacZ (β-galactosidase) gene, so β-galactosidase is not produced and the colonies appear white. Non-recombinant bacteria produce β-galactosidase and form blue colonies.

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